The CFM Printer
The Continuous Flow Microspotter (CFM) prints biomolecules by cycling the samples over spot surfaces and capturing them from solution. Benefits of printing with flow include enhanced concentration from dilute solutions, selective capture directly from crude media, the performance of multi-step assays on a spot by delivering multiple reagents in sequence, and the printing of sensitive molecules because they do not dry out within the enclosed microchannel network.
The CFM uses a network of microchannels to print 48 samples at one time in a 4 x 12 block. Automated fluid handling and positioning systems enable printing of up to 768 unique samples in one run. The system is configured for printing onto standard 1" x 3" slides. However, custom slide holders can be made for different slide types.
The CFM has been used to print a wide variety of biomolecules including DNA, proteins, antibodies, lipids, sugars and cells in array formats that work well with both labeled and label free imaging instruments. When used with label free SPR imaging technologies, CFM printed arrays routinely demonstrate higher sensitivity and lower variation in end point and kinetic data when compared to other microarray printing methods.
System Operation
The following video displays the general setup of the CFM instrument. You should see a grey video screen with controls at the bottom. Press the play arrow in the lower left corner to begin.
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Specifications
Dimensions (w x d x h) |
22" x 22" x 20" |
Weight |
105 lbs. |
Electrical Requirements |
Standard: 110 V 60 Hz |
|
Optional: 230 V 50 Hz |
Sample Loading |
Two standard microtiter plates (96 or 384 well) |
Slide Formats |
Standard: 1" x 3" |
|
Optional: Custom slide mounts available |
System Features and Advantages
